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814005

TSKgel® Octadecyl-NPR (2.5 µm) HPLC Columns

L x I.D. 3.5 cm × 4.6 mm, C18 (octadecyl) phase

Synonym(s):

TSKgel® Reversed Phase HPLC Column (Polymer-based)

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About This Item

UNSPSC Code:
41115700

product name

TSKgel® Octadecyl-NPR HPLC Column, phase C18 (octadecyl), L × I.D. 3.5 cm × 4.6 mm, 2.5 μm particle size

material

stainless steel

product line

TSKgel®

manufacturer/tradename

Tosoh 14005

extent of labeling

1 meq/mL ligand density (estimate)

parameter

≤0.5 M salt concentration
0-100% organic solvent
0-60 °C temperature
1.6 mL/min flow rate
200 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

3.5 cm × 4.6 mm

matrix

polymer particle platform

matrix active group

C18 (octadecyl) phase

particle size

2.5 μm

operating pH

2-12

separation technique

reversed phase

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General description

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. NPR, non-porous resin, columns are prepared from non-porous methacrylate particles of uniform 2.5 micron size, which provides high efficiency separations and fast anaylses of peptides and proteins. The non-porous particle structure limits product isolation to sub-microgram loads.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

Physical form

Shipped in 70% methanol - 30% waters (v/v).

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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