For assay purposes, this product is prepared in 50 mM Potassium Phosphate Buffer, pH 7.5 at 25°C. The material is lyophilized from a solution containing BSA and sodium glutamate. While the solubility has not specifically been tested in sodium phosphate buffer, it is expected to be suitable. Please see the link below to review the assay protocol for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/938/x2252enz.pdf
X2252
Xanthine Oxidase microbial
lyophilized powder, ≥7 units/mg solid
Synonym(s):
Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
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25 UNITS
€114.00
100 UNITS
€349.00
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25 UNITS
€114.00
100 UNITS
€349.00
About This Item
Recommended Products
biological source
microbial
Quality Level
form
lyophilized powder
specific activity
≥7 units/mg solid
mol wt
~160 kDa
technique(s)
inhibition assay: suitable
color
brown
optimum pH
7.5-8.0
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)
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General description
Research area: Cell Cycle
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Application
Xanthine Oxidase microbial has been used:
Biochem/physiol Actions
Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism.[3][5] This enzyme is also implicated in caffeine metabolism.[6] XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid.[7] Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.[8]
Also contains proteolytic activity.
Physical properties
Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Storage and Stability
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
antibody
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
The cytotoxicity of five oxysterols identified in cooked fish, 7-ketocholesterol, 7beta-hydroxycholesterol, cholesterol 5alpha,6alpha-epoxide, cholestanetriol and 4-cholesten-3-one, was investigated in primary cultures of bovine ovarian granulosa cells. Cells were exposed to the oxysterols individually and to a mixture of the same
P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Giuseppe Faudone et al.
Journal of medicinal chemistry, 64(11), 7156-7178 (2021-05-22)
The purine alkaloid caffeine is the most widely consumed psychostimulant drug in the world and has multiple beneficial pharmacological activities, for example, in neurodegenerative diseases. However, despite being an extensively studied bioactive natural product, the mechanistic understanding of caffeine's pharmacological
Kavita R Hegde et al.
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde, 222(3), 194-198 (2008-05-24)
Oxidative stress is considered to be a significant risk factor in the pathogenesis of many eye diseases associated with aging. The purpose of this study was to investigate the susceptibility of retinal tissue to reactive oxygen species (ROS)-induced damage, and
Protocols
The appearance of uric acid is measured at 293nm by spectrophotometry.
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Guten Tag, in welchem Lösungsmittel und bis zu welcher Konzentration ist das Enzym gut löslich? Kann Natrium Phosphat Puffer verwendet werden? Danke.
1 answer-
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