immunohistochemistry: suitable indirect immunofluorescence: 2.5-5.0 μg/mL using mouse 3T3 cells or rat NRK cells western blot: 1.0-2.0 μg/mL using histones isolated from human HeLa cells.
peptide containing methylated lysine 20 of human Histone H4, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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