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MAK155

Sigma-Aldrich

Fluorometric Sphingomyelinase Assay Kit

sufficient for 200 fluorometric tests (red fluorescence)

Synonym(s):

Neutral Sphingomyelinase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 fluorometric tests (red fluorescence)

detection method

fluorometric

relevant disease(s)

cancer; rheumatological diseases

storage temp.

−20°C

Gene Information

human ... SMPD2(6610) , SMPD3(55512) , SMPD4(55627)
mouse ... SMPD2(20598) , SMPD3(58994) , SMPD4(77626)
rat ... SMPD2(83537) , SMPD3(94338) , SMPD4(303790)

General description

Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress. Mutations in is associated with metabolic disorders like Niemann-Pick disease B.

Suitability

This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.

Principle

The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.

Signal Word

Danger

Hazard Statements

H302,H317,H318,H334,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sphingomyelinases: enzymology and membrane activity.
Go?i F M and Alonso A
Febs Letters, 531(1), 38-46 (2002)
Sphingomyelinases: their regulation and roles in cardiovascular pathophysiology.
Pavoine C and Pecker F
Cardiovascular Research, 82(2), 175-183 (2009)
Secretory sphingomyelinase in health and disease.
Kornhuber J, et al.
Biological Chemistry, 396(6-7), 707-736 (2015)

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