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M7145

Sigma-Aldrich

MEM Non-essential Amino Acid Solution (100×)

Without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

MEM NEAA

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100 ML
€34.70

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100 ML
€34.70

About This Item

UNSPSC Code:
12352207
eCl@ss:
32160406
NACRES:
NA.75

€34.70

Please contact Customer Service for Availability
Request a Bulk Order

Product Name

MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

Eagle′s minimum essential medium (MEM) is a synthetic culture medium, which is composed of balanced salt solutions, and amino acids and vitamins. These components are essential for the growth of cultured cells. MEM might be supplemented with non-essential amino acids, to make MEM non-essential amino acid solution. The non-essential amino acids include L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and L-glutamic acid.[1]

Application

Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. MEM Non-essential Amino Acid Solution supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
MEM Non-essential Amino Acid Solution has been used in Dulbecco′s minimum essential media for the maintenance of mammalian cells.[2] It has been supplemented in medium prepared for oocyte maturation.[3][4]

Biochem/physiol Actions

Studies on hybridoma culture shows that addition of Eagle′s minimum essential medium (MEM) non-essential amino acid leads to enhanced membrane stability, during death phase of the culture. This also results in increased antibody secretion during death phase, and decreased lactate concentration.[5]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBM1904
RNBM0563
RNBM0097
RNBL7298
RNBL4185

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Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Cell Culture
Jakoby WB
Methods in Enzymology, 58 (1979)
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus-oocyte complexes.
Hashimoto S et al.
Molecular Reproduction and Development, 57, 353-353 (2000)
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Questions

1–4 of 4 Questions  

  1. 안녕하세요. Lot. RNBL4088 제품 유통기한 알 수 있을까요?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. What is the concentration of Product M7145, MEM Non-essential Amino Acids, 100X solution?

    1 answer

    1. M7145 is a 100X solution of amino acids (Ala, Asn, Asp, Glu, Gly, Pro and Ser). The concentration of each of the seven amino acid at 100X is 10 mM; therefore at 1X, the concentration of each of them is 0.1 mM.

      Please see the component chart for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/334/224/m7145for.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can I store Product M7145, MEM Non-essential Amino Acids, 100X solution at -20°C?

    1 answer

    1. Product M7145 is a 100X solution of non-essential amino acids that should be stored at 2-8 °C for maximum stability. If the product is properly stored and handled, we guarantee the product for one year after it is received. We have not tested the stability of the product at -20°C for long term storage, so we do not recommend aliquoting and freezing the product. It is likely that storage at -20°C will cause precipitation of some of the amino acids upon thawing. If precipitation occurs, the product should not be used.

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