Skip to Content
Merck
All Photos(1)

Key Documents

GEE70092Y

Shrimp Alkaline Phosphatase

Cytiva E70092Y, pack of 500 U

Sign Into View Organizational & Contract Pricing

Select a Size

1 PKG
€134.00

€134.00

Please contact Customer Service for Availability
All Photos(1)

Select a Size

Change View
1 PKG
€134.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

€134.00

Please contact Customer Service for Availability

biological source

shrimp

form

liquid

mol wt

155 kDa

packaging

pack of 500 U

manufacturer/tradename

Cytiva E70092Y

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Shrimp alkaline phosphatase (SAP) has a high specific activity and is a heat-labile alkaline phosphatase.[1] It can be inactivated by a short rise in temperature to 65 °C. SAP is a 155,000Da homodimeric protein with dimensions of about 95 Å×65 Å×50 Å and mol. wt of 155,000.[1][2]
Completely and irreversibly inactivated in Tris buffers at pH 8.0-8.5 by heating for 15 min at 65°C. No further treatment is necessary.

Biochem/physiol Actions

Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs).[3] The enzyme activity can be completely inhibited by ethylenediaminetetraacetic acid (EDTA), but the activity can be restored to a large degree by zinc.[4]
Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs).[1] The enzyme activity can be completely inhibited by ethylenediaminetetraacetic acid (EDTA), but the activity can be restored to a large degree by zinc.[2]

Features and Benefits

  • Removing 5′-phosphates from DNA and RNA.
  • Easily inactivated by heat.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Storage Class Code

12 - Non Combustible Liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): a dimeric enzyme with catalytically active subunits.
Olsen R L, et val.
Comparative Biochemistry and Physiology. B, Comparative Biochemistry, 99(4), 755-761 (1991)
The 1.9 ? crystal structure of heat-labile shrimp alkaline phosphatase.
de Backer M, et al.
Journal of Molecular Biology, 318(5), 1265-1274 (2002)
The 1.9 A crystal structure of heat-labile shrimp alkaline phosphatase.
de Backer M, et al.
Journal of Molecular Biology, 318, 1265-1274 (2002)
Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): A dimeric enzyme with catalytically active subunits
Olsen RL, et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 99, 755- 761 (2003)
Exploring alternative pathways for the in vitro establishment of the HOPAC cycle for synthetic CO2 fixation.
McLean, et al.
Science Advances, 9, eadh4299-eadh4299 (2023)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service