G8415

L-Glutamic acid

98.5-100.5%, suitable for cell culture, non-animal source, meets EP testing specifications

• Synonym(s): (S)-2-Aminopentanedioic acid, Glu
• Linear Formula: HO₂CCH₂CH₂CH(NH₂)CO₂H
• CAS Number: 56-86-0
• Molecular Weight: 147.13
• Beilstein: 1723801
• EC Number: 200-293-7
• MDL number: MFCD00002634
• UNSPSC Code: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 24895340
• NACRES: NA.26

Properties

• Product Name: L-Glutamic acid, from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%
• biological source: non-animal source
• Quality Level: 300
• Agency: meets EP testing specifications
• Assay: 98.5-100.5%
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• mp: 205 °C (dec.) (lit.)
• solubility: 1 M HCl: 100 mg/mL
• density: 1.54 g/cm³ at 20 °C
• anion traces: chloride (Cl^-): ≤200 ppm; sulfate (SO₄^2-): ≤200 ppm
• cation traces: As: ≤1 ppm, passes test; Fe: ≤10 ppm, passes test; NH₄⁺: ≤200 ppm, passes test
• application(s): pharmaceutical (small molecule)
• SMILES string: N[C@@H](CCC(O)=O)C(O)=O
• InChI: 1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m0/s1
• InChI key: WHUUTDBJXJRKMK-VKHMYHEASA-N
• Gene Information: human ... CCR2(1231) , GRIA1(2890) , GRIA2(2891) , GRIA4(2893) , GRIK1(2897) , GRIK2(2898) , GRIK3(2899) , GRIK5(2901) , GRIN2B(2904) , GRM2(2912) , SLC1A1(6505) , SLC1A2(6506) ; rat ... Gria1(50592) , Grik1(29559) , Grik2(54257) , Grik4(24406) , Grin2a(24409) , Grm1(24414) , Grm2(24415) , Grm3(24416) , Grm4(24417) , Grm5(24418) , Grm6(24419) , Grm7(81672) , Slc1a2(29482)

Description

Application

L-Glutamic acid has been used as a supplement in for cell culture media to culture hippocampal neurons and hT-PA (human tissue plasminogen activation) producing cell line.[1][2][3]

Biochem/physiol Actions

Glutamic acid, or glutamate, the salt form of glutamic acid, functions as a neurotransmitter and also serves as a precursor to other neurotransmitters such as γ-aminobutyric acid.[4] Glutamic acid also plays a key role in many metabolic pathways, that controls growth, reproduction, maintenance and immunity.[5] It is converted to α-ketoglutarate, a key component of the TCA (tricarboxylic acid) cycle, and a precursor for the biosynthesis of nucleic acids and certain amino acids.[5][6] In cells, glutamine is converted to glutamate by the enzyme glutaminase.[6] Glutamine serves as a source of energy for rapidly dividing cells comprising lymphocytes, enterocytes, macrophages and tumors. Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signaling. It is also known to be associated with the inhibition of apoptosis.[5]

An excitatory amino acid neurotransmitter that is an agonist at all subtypes of glutamate receptors (metabotropic, kainate, NMDA, and AMPA).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves