E0760

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

• Synonym(s): ECGS, Endothelial Mitogen
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Endothelial cell growth supplement from bovine pituitary, ECGS, suitable for cell culture, BioReagent
• biological source: bovine pituitary glands
• Quality Level: 200
• product line: BioReagent
• form: powder
• potency: 2-300 μg/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• packaging: pkg of 15 mg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤20 EU/vial (LAL test)
• color: brown to tan
• solubility: water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
• storage temp.: 2-8°C

Description

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands[1] containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

• as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture[2]
• as a supplement in medium 199 for endothelial cell culture[3]
• as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12[4]
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture[5]

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H361fd
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable