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Product Name
LB83, 98062328
biological source
human blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human lymphocytes, Xeroderma pigmentosum, EBV-transformed
Cell Line Description
Established by EBV transformation of mononuclear cells from the peripheral blood of a 15 year old female. The patient has been diagnosed with Xeroderma pigmentosum (XP) complementation group G, showed no skin cancer or neurologic abnormalities and reduced NK activity. XP is a relatively rare recessively inherited disease with DNA repair deficiency. Fibroblasts from the same individual have been designated XP125LO.
Application
DNA repair defect studies, hprt mutation analysis
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%
Other Notes
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