Recommended Products
product name
IEC 18, 88011801
biological source
rat intestine
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Normal rat ileum
Cell Line Description
Derived from normal epithelial cells of the rat ileum. Cells should undergo at least 20 population doublings. The line was established using the same techniques as for IEC6 (Sigma Catalogue number. 88071401). IEC 18 cells grow in small islands and do not reach confluency.
Application
Small bowel biology
Culture Medium
DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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