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Product Name
SV3T3B, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85011409
biological source
mouse embryo
packaging
tube of 5 μg 85011409-DNA-5UG pkg of vial of cells 85011409-1VL
A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (Sigma Catalogue number. 90030801). Demonstrates a high degree of contact inhibition.
Application
Contact inhibition, transformation and virus studies: SV40
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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