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90903

Sigma-Aldrich

Schaeffer and Fulton Spore Stain Solution A

for microscopy

Synonym(s):

Malachite Green Solution according to Schaeffer and Fulton

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100 ML
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100 ML
€24.80

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

€24.80


Please contact Customer Service for Availability

Request a Bulk Order

grade

for microscopy

product line

BioChemika

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy
spore forming microorganisms

SMILES string

[N+](=C4C=CC(=C(c6ccc(cc6)N(C)C)c5ccccc5)C=C4)(C)C.[N+](=C1C=CC(=C(c3ccc(cc3)N(C)C)c2ccccc2)C=C1)(C)C.[O-]C(=O)C(=O)[O-].OC(=O)C(=O)O.OC(=O)C(=O)O

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

InChI key

CNYGFPPAGUCRIC-UHFFFAOYSA-L

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Components

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

replaced by

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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P Manoj Kumar Reddy et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 67(5), 1097-1104 (2013-02-19)
Oxidative decomposition of aqueous organic pollutant malachite green (MG) was studied in a dielectric barrier discharge reactor operated under ambient conditions. Total organic carbon content analysis confirmed the mineralization of the pollutant leading to the formation of carbon dioxide, which
R Sahraei et al.
Environmental monitoring and assessment, 185(7), 5817-5822 (2012-12-04)
A new spectrophotometric method is reported for the determination of nanomolar level of malachite green in surface water samples. The method is based on the catalytic effect of silver nanoparticles on the oxidation of malachite green by hexacyanoferrate (III) in
Yang Wu et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 83(2), 220-226 (2013-01-11)
Monitoring the trafficking of multiple proteins simultaneously in live cells is of great interest because many receptor proteins are found to function together with others in the same cell. However, existing fluorescent labeling techniques have restricted the mechanistic study of
Liping Wen et al.
Advanced materials (Deerfield Beach, Fla.), 24(46), 6193-6198 (2012-09-29)
A highly efficient and perfectly reversible ionic gate that can be activated by pH or UV light is demonstrated. Switching between the OFF state and the ON state is mainly dependent on the surface charge transition brought about by a
Ji'ai Wang et al.
PloS one, 7(12), e51808-e51808 (2012-12-20)
Malachite green (MG), N-methylated diaminotriphenylmethane, is one of the most common dyes in textile industry and has also been used as an effective antifungal agent. However, due to its negative impact on the environment and carcinogenic effects to mammalian cells

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