The protein named Leukemia-associated RhoGEF (LARG) or alternatively known as Leukemia-associated RhoGEF protein, and encoded by the gene ARHGEF12/KIAA0382/LARG, plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) proteins. Leukemia-associated RhoGEF (LARG) acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Additionally, Leukemia-associated RhoGEF (LARG) interacts with RHOA, PLXNB1 and PLXNB2, and it interacts with IGF1R beta subunit and GCSAM proteins. Leukemia-associated RhoGEF (LARG) is localized to the cytoplasm and membrane. The protein translocates to the membrane upon stimulation. Leukemia-associated RhoGEF (LARG) widely expressed with isoform specific isoforms being found in certain tissues only, such as isoform 2 is primarily found in the intestine and testis.
Immunogen
Epitope: RGSL Domain
GST-tagged recombinant protein corresponding to the RGSL domain of human Leukemia-associated RhoGEF/LARG.
Application
Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1 is a highly specific mouse monoclonal antibody, that targets Leukemia-associated RhoGEF & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Leukemia-associated RhoGEF/LARG in HepG2 and HeLa cells.
Research Category Cell Structure
Research Sub Category Cytoskeletal Signaling
Quality
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Leukemia-associated RhoGEF/LARG in 200 µg of HUVEC cell lysate.
Target description
~ 173 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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