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100 μL
€363.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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antibody form
purified antibody
clone
NaR9
monoclonal
purified by
using protein G
species reactivity
mouse, mouse, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2aκ
UniProt accession no.
Mode of action
| inhibits
General description
Ba/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.
Specificity
Clone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.
Application
Immunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Quality
Evaluated by Immunocytochemistry in Raw264.7 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.
Physical properties
116.41 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG2a in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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