The adhesion and degranulation adaptor protein (ADAP) acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. ADAP modulates the expression of interleukin-2 (IL-2) and it is suggested to be involved in platelet activation. Additionally ADAP is shown to prevent the degradation of SKAP1 and SKAP2 and may play a role in linking T-cell signaling to the remodeling of the actin cytoskeleton. ADAP is expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. At least two isoforms of ADAP are known to exist.
Specificity
This antibody recognizes the C-terminus of ADAP.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human ADAP.
Application
Detect ADAP using this rabbit polyclonal antibody, Anti-ADAP Antibody validated for use in western blotting & ICC.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected ADAP in K562 cells.
Research Category Cell Structure
Research Sub Category Adhesion (CAMs)
Quality
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ADAP in 15 µg of K562 cell lysate.
Target description
~130 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 2 isoforms at ~85 kDa and ~91 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control K562 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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