Bcl-2-modifying factor (Bmf) is a member of the Bcl-2 family containing one BCL2 homology domain 3. Members of this family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell′s actin cytoskeleton. Bmf is constitutively expressed in many tissues. There are 3 isoforms of Bmf. Isoform 1 is mainly expressed in B-lymphoid cells while isoform 2 and 3 are mainly expressed in B-CLL and normal B-cells.
Specificity
This antibody recognizes the N-terminus of Bmf.
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Bmf.
Application
Detect Bcl-2-modifying factor using this rabbit polyclonal antibody, Anti-Bmf Antibody validated for use in western blotting & IHC.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Bmf in HeLa cells.
Quality
Evaluated by Western Blotting in HepG2 and HEK293 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Bmf in 15 µg of HepG2 and HEK293 cell lysates.
Target description
~25 kDa observed
Analysis Note
Control HepG2 and HEK293 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
![Naphtho[1,2-c:5,6-c′]bis[1,2,5]thiadiazole-5,10-diboronic acid bis(pinacol) ester 95%](/deepweb/assets/sigmaaldrich/product/structures/396/334/bb0914db-5c9a-4565-ba76-30dd4bc4ba87/640/bb0914db-5c9a-4565-ba76-30dd4bc4ba87.png)
![[1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)](/deepweb/assets/sigmaaldrich/product/structures/130/734/8846aa26-1858-458a-998d-8c306c13bf0f/640/8846aa26-1858-458a-998d-8c306c13bf0f.png)
