Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

71235

Sigma-Aldrich

pIEx-4 DNA - Novagen

Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Synonym(s):

InsectDirect™ System- pIEx-4 DNA

Sign Into View Organizational & Contract Pricing

Select a Size

20 μG
€595.00

€595.00

Estimated to ship on15 April 2025Details

All Photos(1)

Select a Size

Change View
20 μG
€595.00

About This Item

UNSPSC Code:
41106611
NACRES:
NA.51

€595.00

Estimated to ship on15 April 2025Details

Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.

pIEx-4 contains C-terminal S•Tag and His•Tag coding sequences. For sequencing or PCR of recombinants in pIEx-4, refer to the table below to choose the appropriate primers.

Warning

Toxicity: Standard Handling (A)

Legal Information

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

  1. What are the significant differences between vector pIEx-4 and pIEx-6? The aim is to express a membrane-specific protein without needing any signal peptidase at the 5' end.

    1 answer

    1. The pIEx™-4 DNA contains C-terminal S•Tag and His•Tag coding sequences, whereas the pIEx™-6 DNA contains an amino-terminal His•Tag coding sequence and an optional S•Tag coding sequence at the distal end of the multiple cloning site (MCS).

      Additionally, the pIEx™-6 DNA features a PshA I cloning site that overlaps the cleavage site for the enterokinase (EK) protease, enabling the removal of all amino-terminal vector-encoded sequences by EK digestion, which is not the case for pIEx™-4 DNA.

      Furthermore, neither pIEx™-4 DNA nor pIEx™-6 DNA offers the option of using a signal sequence to secrete the protein of interest into the surrounding medium.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service