DNA Methyltransferase Inhibitor V, RSC133, ( E)-3-(3-(1H-Indol-3-yl)acrylamido)benzamide, ID133, iPSC Induction Enhancer III, Reprogramming Stimulating Compound 133, DNA MTase Inhibitor V
A cell-permeable compound that targets Dnmt1 at the cofactor SAM-binding site and enhances the efficiency of iPSC production from both murine MEF and human foreskin fibroblasts.
A cell-permeable indolylacrylamido-benzamide compound that is reported to target Dnmt1 at the cofactor SAM-binding site and enhance the efficiency of iPSC production from both murine MEF (10 µM starting 5 d post KSOM viral infection) and human foreskin fibroblast (10 µM starting same day as or 5 d post KSOM viral infection) cultures with accompanying cellular Dnmt1 activity reduction, affecting hFF viability only at high concentrations (78% of control post 72 h 100 µM treatment). Also shown to help maintain H9 hESCs in undifferentiated state when cultured in unconditioned media (10 µM).
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Biochem/physiol Actions
Cell permeable: yes
Primary Target Dnmt1
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Use only fresh DMSO for reconstitution.
Other Notes
Lee, J., et al. 2012. Angew. Chem. Int. Engl.51, 12509.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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