200182

Nitrazine Yellow

indicator grade, Dye content 85 %

• Synonym(s): 2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow
• Empirical Formula (Hill Notation): C₁₆H₈N₄Na₂O₁₁S₂
• CAS Number: 5423-07-4
• Molecular Weight: 542.36
• Colour Index Number: 14890
• EC Number: 226-549-8
• MDL number: MFCD00003938
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851961
• NACRES: NA.47

Properties

• grade: indicator grade
• Assay: >90.0% (HPLC)
• form: powder
• composition: Dye content, 85%
• solubility: H₂O: 1 mg/mL
• λmax: 586 nm
• ε (extinction coefficient): ≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;
• InChI key: XBLIFEQTVVSTIM-QIKYXUGXSA-L

Description

Application

Nitrazine Yellow has been used in dye-protein binding assay.[1]

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye.[2] It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy.[2][3] Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.[4]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves