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F5542

Sigma-Aldrich

Fibroblast Growth Factor-Acidic human

FGF-Acidic, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

FGF-1, aFGF

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About This Item

MDL number:
MFCD00081269
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.287 ng/mL

quality

endotoxin tested

mol wt

15.5 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/mg (LAL test)

UniProt accession no.

P05230

storage temp.

−20°C

Gene Information

human ... FGF1(2246)

Related Categories

General description

Fibroblast Growth Factor-Acidic (FGF1) belongs to the FGF family and comprises a nuclear export sequence (NES). FGF1 gene is mapped to human chromosome 5q31.3. FGF1 is highly expressed in the central and peripheral nervous system.

Application

Fibroblast Growth Factor-Acidic human has been used:
  • as a component of growth factor cocktail for treating neural stem cell (NSC) transplants
  • as a component of Human Neural Stem Cell (HNSC) proliferation medium for Neural Tissue Spheres (NTS) propagation
  • as a growth factor component in fibrin matrix to support embryonic day 14 (E14) cells graft survival

Biochem/physiol Actions

Fibroblast Growth Factor-Acidic (FGF1) is a potent mitogenic agent associated with a variety of tissue origins including liver, vasculature, and skin. These include for a wide variety of mesoderm-derived cells including BALB/c 3T3 fibroblasts, capillary and endocardial endothelial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astroglial cells, and adrenal cortex. FGF1 participates in glucose homeostasis, adipose tissue remodeling and is a key transducer of the nuclear receptor, peroxisome proliferator-activated receptor γ (PPARγ). FGF1 is a neuronal cell neurotrophic factor and binds to most of the isoforms of the FGF receptor (FGFR) isoforms. Along with FGF2, FGF1 plays a key role in angiogenesis and favors blood vessel formation in chronic inflammatory diseases and cancer. The FGF1 mutant R50E leads to suppression of its angiogenic potential making it a potential anti-angiogenesis agent.

Physical form

The product is lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1 M NaCl, 5 mM DTT containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The proliferative activity is tested in culture using quiescent NR6R-3T3 fibroblasts.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T Yamashita et al.
Biochemical and biophysical research communications, 277(2), 494-498 (2000-10-18)
We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus ( approximately 24 amino acids), which is a typical signal sequence. As
Zhifeng Huang et al.
Cell reports, 20(7), 1717-1728 (2017-08-17)
The recent discovery of metabolic roles for fibroblast growth factor 1 (FGF1) in glucose homeostasis has expanded the functions of this classically known mitogen. To dissect the molecular basis for this functional pleiotropy, we engineered an FGF1 partial agonist carrying triple
Paul Lu et al.
Cell, 150(6), 1264-1273 (2012-09-18)
Neural stem cells (NSCs) expressing GFP were embedded into fibrin matrices containing growth factor cocktails and grafted to sites of severe spinal cord injury. Grafted cells differentiated into multiple cellular phenotypes, including neurons, which extended large numbers of axons over
Katarzyna Dominika Sluzalska et al.
Cytokine & growth factor reviews (2020-06-02)
Fibroblast growth factors 1 and 2 (FGF1 and FGF2) are mainly considered as ligands of surface receptors through which they regulate a broad spectrum of biological processes. They are secreted in non-canonical way and, unlike other growth factors, they are
Seiji Mori et al.
PloS one, 8(2), e57927-e57927 (2013-03-08)
Fibroblast growth factor-1 (FGF1) and FGF2 play a critical role in angiogenesis, a formation of new blood vessels from existing blood vessels. Integrins are critically involved in FGF signaling through crosstalk. We previously reported that FGF1 directly binds to integrin
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