52819

Rhamnose MacConkey (VTEC O26) Agar

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): RMAC
• UNSPSC Code: 41171609
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 12.0 g/L ; bile salts No. 3, 1.5 g/L ; crystal violet, 0.001 g/L ; neutral red, 0.03 g/L ; peptone, 15.0 g/L ; rhamnose, 20.0 g/L ; sodium chloride, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Basic
• final pH: 7.1±0.2 (25 °C)
• application(s): clinical; environmental; food and beverages; microbiology
• suitability: enterovirulent E. coli (EEC)

Description

Application

This medium is selective for the isolation of the verocytotoxin producing Escherichia coli O26. This strain has been associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). It is based on the MacConkey formulation No.3, where the fermentable carbohydrate of lactose has been substituted for rhamnose. VTEC O26 colonies are not able to ferment the rhamnose so will remain a translucent colour on the medium. All other non-O26 VTEC colonies present are able to ferment Rhamnose and will appear as pink to red colonies. Selectivity of the medium can be increased by adding Cefixime Tellurite (CT) supplement.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3