1.07627

Pseudomonas CFC Selective Supplement

for Pseudomonas spp., pkg of 10 vials, for the preparation of Pseudomonas CFC Selective Agar

• Synonym(s): Pseudomonas Selective Supplement
• UNSPSC Code: 41106212
• NACRES: NA.74

Properties

• description: Pseudomonas CFC/CN agar (base) acc. ISO 13720 and ISO 16266 (1.07620)
• Quality Level: 200
• form: solid
• composition: Cephalothin, 25 mg per vial ; Cetrimide, 5 mg per vial ; Fucidin, 5 mg per vial
• packaging: pkg of 10 vials
• technique(s): microbiological culture: suitable
• pH: 6.2-6.8 (20 °C, 26.2 g/L in H₂O)
• solubility: 26.6 g/L
• application(s): food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: Pseudomonas spp.

Description

General description

The Pseudomonas CFC Selective Supplement is a mixture of 3 different inhibitors in lyophilized form. Cetrimide, Fucidin, and Cephalothin inhibit the accompanying Gram-positive and Gram-negative flora.

Application

Pseudomonas CFC Selective Supplement is an additive for the preparation of Pseudomonas CFC Selective Agar for the detection and enumeration of Pseudomonas spp. from food and animal feed.

Analysis Note

Recovery on test medium
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
≥ 70 %
- Pseudomonas putida ATCC 12633 (WDCM 00117): ≥ 70 %
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): ≥ 70 %
- Pseudomonas fragi ATCC 27362: ≥ 70 %
- Pseudomonas fragi ATCC 4973 (WDCM 00116): ≥ 70 %
- Burkholderia cepacia ATCC 17759: ≥ 70 %
- Proteus mirabilis ATCC 14153: ≤ 0.01 %
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≤ 0.01 %
Colonies
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
greenish
- Pseudomonas putida ATCC 12633 (WDCM 00117): greenish
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): greenish
- Pseudomonas fragi ATCC 27362: milky
- Pseudomonas fragi ATCC 4973 (WDCM 00116): milky
- Burkholderia cepacia ATCC 17759: milky
Incubation: 44 hrs.; 25 °C; aerobic;
Reference: blood-agar (24-48 hrs.; 30 °C; aerobic).

Supplement tested in Pseudomonas selektiv agar (base) art. no. 1.07620.

Safety Information

• Pictograms: GHS05,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H317 - H318 - H334 - H410
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3