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G9880

Sigma-Aldrich

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

Synonym(s):

ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

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About This Item

CAS Number:
Enzyme Commission number:
Beilstein:
3562140
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine heart

Quality Level

form

ammonium sulfate suspension

specific activity

≥75 units/mg protein

mol wt

42 kDa

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

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General description

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

Biochem/physiol Actions

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Suspension in 1.8 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Glutamic-Pyruvic Transaminase 1 Facilitates Alternative Fuels for Hepatocellular Carcinoma Growth?A Small Molecule Inhibitor, Berberine
Guo W, et al.
Cancers, 12(7), 1854-1854 (2020)
Paradoxical Inhibition of Glycolysis by Pioglitazone Opposes the Mitochondriopathy Caused by AIF Deficiency
Benit P, et al.
EBioMedicine, 17, 75?87-75?87 (2017)
Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States
Zechel J, et al.
Journal of Separation Science, 79?98-79?98 null
Martin J J Ronis et al.
Experimental biology and medicine (Maywood, N.J.), 238(2), 151-162 (2013-04-12)
Metabolic syndrome is often accompanied by development of hepatic steatosis and less frequently by non-alcoholic fatty liver disease (NAFLD) leading to non-alcoholic steatohepatitis (NASH). Replacement of corn oil with medium chain triacylglycerols (MCT) in the diets of alcohol-fed rats has
Detection of L-Lactate In Polyethylene Glycol Solutions Confirms Identity of Active Site Ligand in Proline Dehydrogenase Structure
Zhang M and Tanner JJ
Acta Crystallographica Section D, Biological Crystallography, 60(0 5), 985?986-985?986 (2004)

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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