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GPT-RO

Roche

Glutamate-Pyruvate Transaminase (GPT)

from pig heart

Synonym(s):

alanine aminotransferase

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204

biological source

pig heart

Quality Level

form

suspension

specific activity

≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)

packaging

pkg of 1 mL (10105589001 [10 mg])
pkg of 2.5 mL (10737127001 [25 mg])

manufacturer/tradename

Roche

optimum pH

~8.0

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Application

Synthesis of unnatural L-amino acids from α-keto acids.

Quality

Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT

Unit Definition

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

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