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GERPN2108

ECL™ Western Blotting Analysis System

Cytiva RPN2108

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

description

Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White

feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2108

storage temp.

2-8°C

General description

ECL™ Western Blotting System is the world′s most widely used and referenced chemiluminescent immunodetection system.

ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.

Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.

Application

ECL™ Blotting Analysis System has been used in western blotting.
Western Blotting

Features and Benefits

  • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents.
  • Optimized system provides rapid, sensitive results using simple protocols.
  • Designed for use with nitrocellulose and PVDF membranes.
  • High sensitivity: Up to 10 times more sensitive than colorimetric methods.
  • Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
  • Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
  • Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
  • Non-radioactive-no specialized facilities needed
  • Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marie-Theres Zeuner et al.
Biochimica et biophysica acta, 1863(12), 3084-3095 (2016-11-05)
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Water for Protein Electrophoresis & Western Blotting

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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