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P2443

Sigma-Aldrich

Pluronic® F-127

powder, BioReagent, suitable for cell culture

Synonym(s):

Non-ionic copolymer surfactant

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250 G
RM 385.00
1 KG
RM 1,281.00

RM 385.00

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250 G
RM 385.00
1 KG
RM 1,281.00

About This Item

Linear Formula:
(C3H6O·C2H4O)x
CAS Number:
9003-11-6
MDL number:
MFCD00082049
UNSPSC Code:
12161902
PubChem Substance ID:
24897874
NACRES:
NA.75

RM 385.00

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description

Non-ionic
contains 100ppm BHT

Quality Level

200

product line

BioReagent

form

powder

mol wt

~12600 g/mol

technique(s)

cell culture | mammalian: suitable

CMC

950-1000 ppm (~25°C)

transition temp

cloud point >100 °C

HLB

18 - 23

SMILES string

O2C(C2)C.O1CC1

InChI

1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2

InChI key

RVGRUAULSDPKGF-UHFFFAOYSA-N

Related Categories

General description

Pluronicpluronic® F-127, also known as poloxamer 407, is a non-toxic[1] and nonionic copolymer. It contains polyethylene oxide-polypropylene oxide arranged in a triblock structure to form PEO-PPO-PEO symmetric.[2] Surfactant properties of this polymer derived from its amphiphilic nature, allow them to interact with hydrophobic surfaces and biological membranes. [3]

Application

Pluronic® F-127 has been used:

  • as a delivery system for a peptide to the wound site to study the effect of the peptide on wound healing in mice.[4]
  • as a surface coating for PDMS (polydimethylsiloxane) stamps to make them hydrogel repellent.[5]
  • in the preparation of calcium indicator stock solution for measuring neural activities.[6]
  • as a component of phosphate-buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.[7]
  • This non-ionic copolymer surfactant is suitable for use in insect cell culture applications as an antifoaming agent.

Biochem/physiol Actions

Pluronic® F-127 is a thermo-reversible gel that finds its application in different industries due to its high thermosensitivity, biodegradability, and biocompatibility properties.[1] These properties make this polymer suitable for use as a versatile drug carrier.Pluronic® F-127 improves the therapeutic efficiency of drugs by increasing their bioavailability and thermostability.[8] Additionally, it can promote cellular attachment and collagen formation, which facilitates angiogenesis and tissue regeneration. Due to its efficacy, Pluronic F-127 has been utilized in the encapsulation of mesenchymal stem cells (MSCs) to aid in the regeneration of tissues with low levels of vascularity, such as epithelial tissues, adipose tissues, cartilage, tendons, and bone tissues.[1]

Legal Information

Pluronic is a registered trademark of BASF

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000418605
0000418605
0000410620
0000410621
0000402593

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Philippe Marmottant et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(41), 17271-17275 (2009-10-07)
Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If
NeuroCa: integrated framework for systematic analysis of spatiotemporal neuronal activity patterns from large-scale optical recording data
Jang MJ and Nam Y
Neurophotonics, 2(3) (2015)
Federico Ceriani et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(46), E7194-E7201 (2016-11-04)
Spatially and temporally coordinated variations of the cytosolic free calcium concentration ([Ca2+]c) play a crucial role in a variety of tissues. In the developing sensory epithelium of the mammalian cochlea, elevation of extracellular adenosine trisphosphate concentration ([ATP]e) triggers [Ca2+]c oscillations
PLURONIC F127 and ITS applications
Salama AH
Pharmacology, 2, 1393- 1403 (2021)
Connexin43 carboxyl-terminal peptides reduce scar progenitor and promote regenerative healing following skin wounding
Ghatnekar GS, et al.
Regenerative Medicine, 4(2), 205-223 (2009)
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Questions

1–10 of 13 Questions  

  1. Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?

    1 answer

    1. There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:

      Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756

      Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162

      Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853

      These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.

      Helpful?

  2. I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?

    1 answer

    1. The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:

      Helpful?

  3. can I know what is the chain length of each PEO and PPO of this F-127?

    1 answer

    1. Pluronic F 127 corresponds to the structure of Kolliphor P 407.
      This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
      A = 101
      B = 56

      Helpful?

  4. What is the VOC content of this product?

    1 answer

    1. Volatile Organic Compound (VOC) content is not measured for this product.

      Helpful?

  5. Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?

    1 answer

    1. The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.

      Helpful?

  6. What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?

    1 answer

    1. The solution can be sterilized with a 0.22 uM sterile filter.

      Helpful?

  7. Is corn oil soluble in Pluronic PBS gel?

    1 answer

    1. In general, Pluronic acid products are poor solubilizers of oils. However, they may make a good dispersant or emulsifier for corn oil.

      Helpful?

  8. Is Pluronic® F-127 the same chemical as Poloxamer 407 (16758-250G)? CAS appears to be the same

    1 answer

    1. Yes, Pluronic® F-127 is the same chemical as Poloxamer 407 as stated under Depositor Supplied

      Synonyms on PubChem are available at this link: https://pubchem.ncbi.nlm.nih.gov/compound/24751#section=Depositor-Supplied-Synonyms.
      Kindly review each of the specifications and tested applications for each product before making a purchasing decision.

      Helpful?

  9. What is the molecular weight of Pluronic F-127, Product P2443?

    1 answer

    1. The average molecular weight is 12.6 kDa.

      Helpful?

  10. Why has my solution of Pluronic F-127, Product P2443, gelled?

    1 answer

    1. Pluronic F-127 will form gels above 10 °C when used at concentrations of 18-50%. It will re-liquefy when cooled to below 10 °C. The gel should remain stable during autoclaving.

      Helpful?

1–10 of 13 Questions  

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