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Key Documents

C2639

Sigma-Aldrich

Cell Freezing Medium-Serum-free 1×

sterile-filtered, suitable for cell culture

Synonym(s):

Cryopreservation Medium, Serum-Free Freezing Medium

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50 ML
RM 1,768.00

RM 1,768.00

Estimated to ship on02 April 2025

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50 ML
RM 1,768.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

RM 1,768.00

Estimated to ship on02 April 2025

sterility

sterile-filtered

Quality Level

100

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

Related Categories

Application

Cell Freezing Medium-Serum-free 1× has been used as a cryopreservation medium for somatic cell sampling and recovery (SCSR) exfoliated cells cultured in growth media [1] and dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cells grown in serum-free growth medium.[2]
Sigma′s Cell Freezing Medium, Serum-free is a cryopreservation medium which does not contain DMSO or serum.

Other Notes

Ready-to-use formula that does not contain dimethyl sulfoxide or serum.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2551
MKCV5907
MKCT8013
MKCS1649
MKCQ9763

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Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG
Albaugh G P, et al.
Open Medicine : A Peer-Reviewed, Independent, Open-Access Journal, 10(6), 126-150 (2020)
Jeong Soo Park et al.
Protein expression and purification, 70(2), 231-235 (2009-10-13)
Hepatocyte growth factor (HGF) is a potent multi-functional protein that affects morphogenesis, cell migration, organ regeneration, and tumor invasion in various tissues, and has thus been considered to have potential as a therapeutic target in various diseases. In our current

Questions

  1. Hi there, can i use this C2639 Cell Freezing Medium-Serum-free 1× for freezing HEK293 and MCF7 cells? In addition, can i use it to freeze hESC and hiPSC?

    1 answer

    1. This Cell Freezing Medium-Serum-free 1× does not contain dimethyl sulfoxide or serum and may not be suitable for all cell types. It is recommended to follow manufacturers' guidelines or literature for cell freezing. For example:

      - Human kidney (embryonic) 293 cells, Cat. #85120602-1VL: 5% dimethyl sulfoxide and 95% fetal calf serum.
      - MCF7 cells: 95% complete growth medium and 5% dimethyl sulfoxide.
      - Simplicon™ Human iPS cells: pluripotent media with 10% dimethyl sulfoxide.
      - Human embryonic stem cells: base medium with fetalbovine serum and 10% dimethyl sulfoxide.

      For cryopreservation of human embryonic stem cells or induced pluripotent stem cells in serum-free conditions, PluriSTEM™ Freeze Medium (SCM134) is recommended.

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