E4884

Ethylenediaminetetraacetic acid disodium salt dihydrate

ACS reagent, 99.0-101.0%

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894586
• NACRES: NA.77

Properties

• grade: ACS reagent
• Quality Level: 200
• Agency: suitable for SM 4500 - NH3
• Assay: 99.0-101.0%
• form: solid
• reaction suitability: reagent type: chelator
• impurities: ≤0.005% Insoluble matter; ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• pH: 4.0-6.0 (25 °C, 5%)
• mp: 248 °C (dec.) (lit.)
• solubility: H₂O: 100 g/L at 20 °C
• cation traces: Fe: ≤0.01%; heavy metals: ≤0.005% (by ICP-OES)
• application(s): microbiology
• storage temp.: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate[1]
• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)[2]
• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart[3]

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

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Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H332,H373
• Precautionary Statements: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves