Proteins, mixture

Materiales

Standard

Referencia del producto

Descripción

Precios

Trypsin inhibitor from Glycine max (soybean)

Isoelectric focusing marker, pI 4.6

Trypsin inhibitor from Glycine max (soybean)

For use as a marker in SDS-PAGE, BioReagent

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

Trypsin inhibitor from Glycine max (soybean)

powder, BioReagent, suitable for cell culture

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Myoglobin from equine heart

Isoelectric focusing marker, pI (1) 6.8, (2) 7.2

Myoglobin from human heart

≥95% (SDS-PAGE), buffered aqueous glycerol solution

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

Conalbumin from chicken egg white

Iron complex

Conalbumin from chicken egg white

BioReagent, suitable for cell culture

Conalbumin from chicken egg white

Substantially iron-free

Trypsin inhibitor from Phaseolus limensis (lima bean)

Type II-L, crude powder

Trypsin inhibitor from Glycine max (soybean)

solution, sterile-filtered, suitable for, suitable for cell culture

Myoglobin from equine skeletal muscle

95-100%, essentially salt-free, lyophilized powder

Trypsin inhibitor from chicken egg white

Type III-O (free of ovoinhibitor)

Trypsin inhibitor from chicken egg white

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder, ~10000 U/mg

Myoglobin from equine skeletal muscle

BioUltra, 95-100% (SDS-PAGE)

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

CONDITIONS

column

Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)

mobile phase

[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)

flow rate

0.5 mL/min

detector

UV, 280 nm

injection

10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

Descripción

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS