Saltar al contenido
Merck
Todas las fotos(1)

Documentos

T9378

Sigma-Aldrich

Trypsin inhibitor

crude powder

Sinónimos:

Trypsin Inhibitor from lima beans

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9035-81-8
EC Number:
232-906-9
MDL number:
MFCD00082102
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

biological source

Phaseolus limensis (lima bean)

type

Type II-L

form

crude powder

mol wt

9 kDa

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma
  • in the termination of proteolytic digestion in rabbit skeletal muscles
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Physical properties

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

G Bellon et al.
Artery, 7(4), 290-302 (1980-01-01)
A serine protease active on insoluble elastin at neutral pH has been isolated from human aortic media employing a Lima-bean trypsin inhibitor - Sepharose column. It is also hydrolyzed Suc (Ala)3 pna and casein but was found inactive against Benzoyl-Tyr-pna
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case
Debreczeni J, et al.
Acta crystallographica. Section D, Structural biology, 59(2), 393-395 (2003)
Malik Shoaib Ahmad et al.
Proteins, 91(1), 22-31 (2022-08-05)
Bovine pancreatic trypsin was crystallized, in-complex with Lima bean trypsin inhibitor (LBTI) (Phaseolus lunatus L.), in the form of a ternary complex. LBTI is a Bowman-Birk-type bifunctional serine protease inhibitor, which has two independent inhibitory loops. Both of the loops
A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis
Lledias F, et al.
The Protein Journal, 36(4), 308-321 (2017)
Shark skeletal muscle tropomyosin is a phosphoprotein
Hayley M, et al.
Journal of Muscle Research and Cell Motility, 29(2-5), 101-107 (2008)
Ver todos los artículos relacionados

Artículos

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocolos

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Chromatograms

application for HPLC

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico