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Discovery® HS F5 (3 µm) HPLC Columns

L × I.D. 5 cm × 4 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Columna para HPLC Discovery® HS F5, 3 μm particle size, L × I.D. 5 cm × 4 mm

material

stainless steel column

agency

suitable for USP L43

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

5 cm × 4 mm

surface area

300 m2/g

surface coverage

4 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

3 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

Características de la Discovery® HS F5:
• Selectividad única
• Retención similar a la C18 (algunas veces requiere una fase móvil más fuerte)
• Excelente forma del pico
• Estable, escaso sangrado en separaciones de CL/EM (LC/MS)
• Separaciones modulables de 3 a 10μm
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Features and Benefits

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificados de análisis (COA)

Lot/Batch Number
291851-01
291850-01
291849-01
291848-01
291088-01

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Jennie Wang et al.
Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)
An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Lucie Havlíková et al.
Talanta, 76(3), 597-601 (2008-07-01)
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
C Desmortreux et al.
Journal of chromatography. A, 1216(42), 7088-7095 (2009-09-15)
Separation of furocoumarins has become of a great interest for cosmetic industry and human health, since the recent directive of the European Union. Furocoumarins are a class of compounds presenting varied substituents linked mainly in two positions to an identical
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