Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Sinónimos:
biomass viability assay, sulforhodamine B, total biomass assay
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
1 KIT
MXP 7,483.00
MXP 7,483.00
Check Cart for Availability
Seleccione un Tamaño
Cambiar Vistas
1 KIT
MXP 7,483.00
About This Item
Código UNSPSC:
12352207
NACRES:
NA.84
MXP 7,483.00
Check Cart for Availability
Productos recomendados
Formulario
liquid
uso
1 kit sufficient for 1,000 tests
envase
pkg of 1 kit
condiciones de almacenamiento
dry at room temperature
λmáx.
565 nm
aplicaciones
cell analysis
detection
método de detección
colorimetric
temp. de almacenamiento
room temp
Descripción general
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Aplicación
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein.[1][2] It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines.[3] Absorbance of dye is measured at a wavelength of 565nm.
Acciones bioquímicas o fisiológicas
Dye binds to cellular protein and is then solubilized in base.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Código de clase de almacenamiento
3 - Flammable liquids
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets
Miranda G, et al.
Neuro-Oncology, 15(2), 172-188 (2012)
Immune expression and inhibition of heat shock protein 90 in uveal melanoma
Faingold D, et al.
Clinical Cancer Research, 14(3), 847-855 (2008)
Artículos
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
-
Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 answer-
Helpful?
-
-
When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 answer-
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
