In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible
application
In Vitro Toxicology Assay Kit, Resazurin based has been used:
in cell viability assay of human primary fibroblasts[1]
to determine the cell proliferation rate in murine pre-osteoblasts[2]
to determine the metabolic activity of microfluidic and monolayer cultures[3]
Biochem/physiol Actions
Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)
A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Ecotoxicology and environmental safety, 25(1), 79-89 (1993-02-01)
The dehydrogenase assay with resazurin as an oxidoreduction dye was used for monitoring the performance of six laboratory-scale sewage treatment plants. The dehydrogenase activity measured proved to be a reliable monitoring parameter, although it did not always positively correlate with
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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