Unfortunately, there is no protocol available for this product. However, see the link below for a publication that may be helpful
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760808/
SRP6371
Aldose Reductase human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Sinónimos:
ADR, AKR1B1, ALDR1, ALR2, AR, Aldo-keto reductase family1, member B1
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About This Item
Código UNSPSC:
12352204
NACRES:
NA.32
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Ensayo
≥95% (SDS-PAGE)
Formulario
liquid
mol peso
35.8 kDa (316 aa, 1-316 aa)
envase
pkg of 100 μg
concentración
1 mg/mL
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... AKR1B1(231)
Descripción general
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily. Recombinant aldose reductase (AKR1B) protein was expressed in Escherichia coli and purified by using conventional chromatography techniques. It is expressed in eye and kidney, but predominant in placenta.[1] The AKR1B1 gene is mapped to human chromosome 7q33.[2]
Acciones bioquímicas o fisiológicas
Aldose reductase (AKR1B1) catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.[3][4] AKR1B1 is also involved in cytokine-mediated generation of prostaglandin F2α (PGF2α) in preadipocytes.[5] It participates in diabetes-associated mitochondrial disorder and damage via the activation of p53.[6] AKR1B1 is associated with drug resistance mechanism in cancer-derived cell lines. It is also known to mediate cellular processes linked to carcinogenesis such as EMT (epithelial–mesenchymal transition), inflammation and angiogenesis.[7] Aldose reductase mediates glucose metabolism in placenta and osmotic control in eye and kidney.[1]
Forma física
1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
The human aldose reductase AKR1B1 qualifies as the primary prostaglandin F synthase in the endometrium.
Bresson E, et al.
The Journal of Clinical Endocrinology and Metabolism, 96, 210-219 (2011)
Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.
Michaud A, et al.
PLoS ONE, 9, e90861-e90861 (2014)
The crystal structure of the aldose reductase.NADPH binary complex.
Borhani DW, et al.
The Journal of Biological Chemistry, 267, 24841-24847 (1992)
Expression of the Aldo-Ketoreductases AKR1B1 and AKR1B10 in Human Cancers.
Laffin B and Petrash JM
Frontiers in Pharmacology, 3, 104-104 (2012)
Aldose reductase-mediated phosphorylation of p53 leads to mitochondrial dysfunction and damage in diabetic platelets.
Tang WH, et al.
Circulation, 129, 1598-1609 (2014)
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