SBR00022

Blasticidin S Ready Made Solution

10 mg/mL (20 mM HEPES), 0.22 μm filtered

• Fórmula empírica (notación de Hill): C₁₇H₂₇N₈O₅Cl
• Peso molecular: 458.90
• Código UNSPSC: 12352106
• NACRES: NA.76

Propiedades

• Nivel de calidad: 200
• esterilidad: 0.22 μm filtered
• Ensayo: ≥98% (HPLC)
• Formulario: liquid
• concentración: 10 mg/mL (20 mM HEPES)
• color: colorless to faint yellow
• espectro de actividad antibiótica: fungi
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: −20°C
• cadena SMILES: CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
• InChI: 1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H
• Clave InChI: YQXYQOXRCNEATG-UHFFFAOYSA-N

Descripción

Descripción general

Blasticidin S, originally isolated from S. griseochromogenes, is a bacterial metabolite renowned for its antibacterial and fungicidal activities. This compound serves as a potent inhibitor of protein synthesis, exhibiting activity against various microorganisms, including bacteria (B. subtilis, S. lutea, E. coli, P. fluorescens, and M. tuberculosis), tumor cell lines, and nematodes.

In research, Blasticidin S has become a valuable tool, notably as a marker for strain manipulations. Recent applications involve the use of Blasticidin S as a selection agent for cells carrying plasmids conferring blasticidin resistance. The resistance is mediated by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus). These genes produce enzymes that catalyze the hydrolytic deamination of the cytosine moiety in blasticidin S, leading to the formation of a non-toxic deaminohydroxy derivative. This resistance mechanism is crucial in various studies involving genetic manipulations and selections in cell biology and biochemical research.

Aplicación

Blasticidin S has been:

• studies to have fungicidal properties and prevents rice blast disease.[1]
• used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Blasticidin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells.[2][3]
• used to study protein synthesis at the level of peptide bond formation.

Acciones bioquímicas o fisiológicas

Mode of Action: It inhibits protein synthesis in bacteria and eukaryotes.[4] Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation.[4][5]

Antimicrobial Spectrum: Active against mycobacteria, several Gram-positive and Gram-negative bacteria

Características y beneficios

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology and Biochemical research

Otras notas

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Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H301
• Consejos de prudencia: P264 - P270 - P301 + P310 - P405 - P501
• Clasificaciones de peligro: Acute Tox. 3 Oral
• Código de clase de almacenamiento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable