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N7265

Sigma-Aldrich

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Sinónimos:

NAD(P)H:Nitrate oxidoreductase

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2 UNITS
MXP 2,492.00
5 UNITS
MXP 4,007.00
10 UNITS
MXP 8,648.00

MXP 2,492.00

Fecha estimada de envío24 de marzo de 2025

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2 UNITS
MXP 2,492.00
5 UNITS
MXP 4,007.00
10 UNITS
MXP 8,648.00

About This Item

Número de CAS:
9029-27-0
Comisión internacional de enzimas:
1.7.1.2 (BRENDA, IUBMB)
MDL number:
MFCD00131725
UNSPSC Code:
12352204
NACRES:
NA.54

MXP 2,492.00

Fecha estimada de envío24 de marzo de 2025

Solicitar un pedido a granel

form

lyophilized powder

Quality Level

200

specific activity

≥300 units/g solid

shipped in

wet ice

storage temp.

−20°C

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General description

Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.[1]

Biochem/physiol Actions

Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.[1]

Caution

light and moisture sensitive

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000347507
0000351286
0000347510
0000347509
0000331250

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E Duncanson et al.
Molecular & general genetics : MGG, 236(2-3), 275-282 (1993-01-01)
Eleven green individuals were isolated when 95000 M2 plants of barley (Hordeum vulgare L.), mutagenised with azide in the M1, were screened for nitrite accumulation in their leaves after nitrate treatment in the light. The selected plants were maintained in
P Hilliquin et al.
Arthritis and rheumatism, 40(8), 1512-1517 (1997-08-01)
Nitric oxide (NO) is a free radical involved in inflammation and immune reactions. The presence of NO is usually assessed by assaying its degradation products, nitrite and nitrate. NO binds to thiol-containing proteins to form S-nitrosoproteins (S-NP). The aim of
E Fernández et al.
Biochimica et biophysica acta, 657(1), 1-12 (1981-01-15)
In vitro complementation of the soluble assimilatory NAD(P)H-nitrate reductase (NAD(P)H:nitrate oxidoreductase, EC 1.6.6.2) was attained by mixing cell-free preparations of Chlamydomonas reinhardii mutant 104, uniquely possessing nitrate-inducible NAD(P)H-cytochrome c reductase, and mutant 305 which possesses solely the nitrate-inducible FMNH2- and
Nilesh Dharajiya et al.
The Journal of allergy and clinical immunology, 119(3), 646-653 (2007-03-06)
Ragweed extract (RWE) contains NADPH oxidases that induce oxidative stress in the airways independent of adaptive immunity (signal 1) and augment antigen (signal 2)-induced allergic airway inflammation. To test whether inhibiting signal 1 by administering antioxidants inhibits allergic airway inflammation
P C Allen et al.
Poultry science, 76(6), 822-827 (1997-06-01)
Two experiments were conducted to determine effects of diets containing n-3 fatty acids (n-3FA) from whole as well as ground flaxseed on the performance of broilers during coccidia infections. Diets were fed from 1 d of age through 3 wk
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Questions

1–2 of 2 Questions  

  1. How much does 2units weigh?

    1 answer

    1. The amount of solid provided will depend on the enzymatic activity reported on the lot-specific Certificate of Analysis. For example, lot SLCR1079 has the enzymatic activity value of 481 units per gram, thus the 2-unit package size would contain roughly 4.16 ug of solid.

      Please view the sample Certificate of Analysis below to see the enzymatic activity:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/N7265-BULKSLCR1079.pdf

      Helpful?

  2. Guten Tag, benötige ich für die Reaktion denn nicht auch das FAD ?

    1 answer

    1. FAD, Flavin Adenine Dinucleotide, is a component used in determining the activity of Nitrate Reductase. See the link below to review the enzymatic assay protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/257/754/n7265enz.pdf

      Helpful?

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