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Merck

N2250

Sigma-Aldrich

Naphthol AS-BI phosphate disodium salt hydrate

fluorogenic, ≥96.0% (HPLC), powder

Sinónimos:

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

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About This Item

Fórmula empírica (notación de Hill):
C18H13BrNNa2O6P · xH2O
Número de CAS:
Peso molecular:
496.16 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)

assay

≥96.0% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O

InChI

1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2

InChI key

WNPKMQCBZKBNOS-UHFFFAOYSA-L

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General description

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.

application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrates

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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