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MAK431

Sigma-Aldrich

Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)

sufficient for 100 colorimetric tests

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About This Item

aplicaciones

pharmaceutical

método de detección

colorimetric

enfermedades relevantes

genitourinary disorders; cancer

temp. de almacenamiento

−20°C

Descripción general

Methionine Adenosyltransferases (MATs), also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the trans-sulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease.

Características y beneficios

Methionine Adenosyltransferase Activity Assay Kit:
  • Compatible with high-throughput handling systems.
  • Limit of quantification of 2 mU MAT activity per well.
  • Homogeneous assay.
  • Does not require complicated sample processing.

Idoneidad

The kit is suitable for the rapid assessment of MAT activity in human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates and cultured cell lysates (adherent or suspension cells) or heterologously expressed recombinant MAT preparations.

Principio

The Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at A570 nm.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

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