Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
M9274
Medio basal Murashige y Skoog
suitable for plant cell culture, with sucrose and agar
Sinónimos:
Medio basal MS
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1 L
MXP 364.00
10 L
MXP 1,271.00
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1 L
MXP 364.00
10 L
MXP 1,271.00
About This Item
Productos recomendados
Nivel de calidad
Formulario
powder
técnicas
cell culture | plant: suitable
aplicaciones
agriculture
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Aplicación
Variante de la fórmula
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
Cantidad
Formulated to contain 42.4 grams of powder per liter of medium.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Ox. Sol. 3
Código de clase de almacenamiento
5.1B - Oxidizing hazardous materials
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Would an incorrect pH level affect the setting of the agar?
1 answer-
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 answer-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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