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M7024

Sigma-Aldrich

Murashige and Skoog Shoot Multiplication Medium A

powder, suitable for plant cell culture

Sinónimos:

MSMA

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1 L
MXP 305.00
10 L
MXP 1,149.00

MXP 305.00


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1 L
MXP 305.00
10 L
MXP 1,149.00

About This Item

Código UNSPSC:
12352207
NACRES:
NA.72

MXP 305.00


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Formulario

powder

Nivel de calidad

técnicas

cell culture | plant: suitable

aplicaciones

agriculture

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Aplicación

Murashige and Skoog Shoot Multiplication Medium A is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Variante de la fórmula

With the macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP as described by Huang and Murashige (1976) TCA Manual.

Media Formulation

Nota de preparación

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M7024 is a variant media) with macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP.

Pictogramas

Flame over circle

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Ox. Sol. 3

Código de clase de almacenamiento

5.1B - Oxidizing hazardous materials

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Plant tissue culture media: major constituents, their preparation and some applications.
Huang L, Murashige T.
Tissue Antigens, 3, 539-548 (1976)
R H Smith et al.
In vitro, 13(5), 329-334 (1977-05-01)
Defined in vitro conditions for callus initiation by Gossypium arboreum L. were determined, and different tissues were evaluated as explant sources, Environmental conditions tested included light versus dark, and low light versus high light. Different nutrient media as well as
Nor Azlina Hasbullah et al.
Pakistan journal of biological sciences : PJBS, 11(11), 1449-1454 (2008-09-27)
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds
Akasaka et al.
Plant science : an international journal of experimental plant biology, 156(2), 169-175 (2000-08-11)
Bud primordia were induced from leaf segments, which were harvested from young seedlings of Spanish type peanut (Arachis hypogaea L. cv. Chico), on 0.8% agar-solidified medium containing Murashige and Skoog (MS) basal salts supplemented with B5 vitamins, 1 mg/l NAA
Regulation of lycopene formation in cell suspension culture of VFNT tomato (Lycopersicon esculentum) by CPTA, growth regulators, sucrose, and temperature
Robertson GE, Mahoney NE
Journal of Experimental Botany, 46, 667-673 (1995)

Questions

  1. In the description of M7024, it is written that the medium, in addition to macro and micronutrients, contains sucrose, IAA and Kinetin. It is also described it contains agar, but the medium did not solidify. What percentage of agar in the product?

    1 answer
    1. This product does not contain Agar. A link to the plant media formulations can be found in the DESCRIPTION section of the product page. Please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

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