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Merck

M1018

Sigma-Aldrich

Medio esencial mínimo Eagle

With Hanks′ salts, ʟ-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

MEM

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10 L
MXP 1,054.00

MXP 1,054.00


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10 L
MXP 1,054.00

About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

MXP 1,054.00


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Nombre del producto

Medio esencial mínimo Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Cantidad

Formulated to contain 10.7 grams of powder per liter of medium.

Reconstitución

Supplement with 0.35 g/L sodium bicarbonate.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Artículos

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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