CWA-1186

Anti-Human CD98 (MEM-108) ColorWheel^® Dye-Ready mAb

• Sinónimos: Large neutral amino acids transporter small subunit 1;4F2 light chain;4F2 LC;4F2LC;CD98 light chain;Integral membrane protein E16;E16;L-type amino acid transporter 1;hLAT1;Solute carrier family 7 member 5;y+ system cationic amino acid transporter
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: MEM-108, monoclonal
• Línea del producto: ColorWheel^®
• Formulario: lyophilized
• mol peso: calculated mol wt 55.01 kDa
• purificado por: using Protein A
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable
• isotipo: IgG1
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_003477.4
• Nº de acceso UniProt: Q01650
• compatibilidad: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• categoría alternativa más sostenible: Aligned,
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... SLC7A5(8140)

Descripción

Descripción general

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidad

Clone MEM-108 is a mouse monoclonal antibody that detects CD98. It targets an epitope within the extracellular domain.

Inmunógeno

Burkitt′s lymphoma Raji cell line.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) gated on monocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on monocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1186, Anti-Human CD98 (MEM-108) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on monocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1186, Anti-Human CD98 (MEM-108) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Compatibilidad

Requires ColorWheel^® Dye (Sold Separately)

Descripción de destino

Large neutral amino acids transporter small subunit 1 (UniProt: Q01650;also known as 4F2 light chain;4F2 LC;4F2LC;CD98 light chain;Integral membrane protein E16;E16;L-type amino acid transporter 1;hLAT1;Solute carrier family 7 member 5;y+ system cationic amino acid transporter) is encoded by the SLC7A5 (also known as CD98LC;LAT1;MPE16) gene (Gene ID: 8140) in human. CD98 is a multi-pass membrane glycoprotein with seven cytoplasmic domains;twelve transmembrane domains;and six extracellular domains. Its expression is observed in the syncytiotrophoblast layer in the placenta;adult lung;liver;brain;bone marrow;testis;and in resting lymphocytes and monocytes. Lower levels of expression is observed in several other tissues. During gestation;its expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. CD98 has two major biochemical functions. Its heavy chain binds to cytoplasmic tails of integrin-β chains and mediates adhesive si

Forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitución

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

Información legal

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable