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Jurkat E6.1 Cell Line human

88042803, human blood (leukemic T-cell lymphoblast), Not specified

Sinónimos:

Jurkat Cells, Jurkat Clone E6-1 Cells

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About This Item

UNSPSC Code:
41106514
biological source:
human blood (leukemic T-cell lymphoblast)
growth mode:
Suspension
karyotype:
Pseudodiploid, modal No 46
morphology:
Not specified
products:
Interleukin 2 (IL-2), human alpha interferon
receptors:
Not specified
relevant disease(s):
cancer
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Nombre del producto

Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)

biological source

human blood (leukemic T-cell lymphoblast)

growth mode

Suspension

karyotype

Pseudodiploid, modal No 46

morphology

Not specified

products

Interleukin 2 (IL-2), human alpha interferon

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Categorías relacionadas

Cell Line Origin

Human leukaemic T cell lymphoblast

Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Application

IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate[1]
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components[2]
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro[3]

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Protocolos

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Questions

1–2 of 2 Questions  

  1. Does this particular batch of Jurkat E6.1 express CD28?

    1 answer

    1. The Jurkat E6.1 cell line is a banked product of ECACC and is tested sterility, viability, mycoplama, and identity. It is not tested for any specific expression. The depositor listed in Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford. The line has not been further modified. Various publications suggest that the E6.1 does express CD28, however we cannot validate these claims. It may be helpful to refer to the literature for further information.

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  2. I am interested in Jurkat cells; product number 88042803 appears to be a vial of these cells for culturing. But why is the extension '-DNA-5UG' in the product number? Does this mean that it is not cells but 5 µg DNA isolated from Jurkat cells???

    1 answer

    1. To order the actual cells, order the SKU with the -1VL suffix, 88042803-1VL. This SKU is for the Jurkat E6.1 cells that can be propagated and maintained in culture. The other SKUs listed there are preparations of DNA, RNA and cDNA from this cell line.

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