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D5796

Sigma-Aldrich

DMEM - high glucose

With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

DME, DMEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

Este medio de glucosa DMEM-Hi es un medio completo 1x sin factores añadidos (modificaciones comunes) como L-alanil-L-glutamina, HEPES o piruvato sódico. Difiere de la formulación DMEM-Hi original en que se sustituye la piridoxina por el piridoxal. El piridoxal es un componente inestable de los medios.

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Optional

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CLS430769

Corning® bottle-top vacuum filter system, cellulose acetate membrane, pore size 0.22 μm, membrane area 33.2 cm2, filter capacity 500 mL

supplement

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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