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293T cell line human

293T cell line human

12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

Sinónimos:

293T cell line human, 293 T, 293tsA1609neo, 293tsA1609neo Cells, HEK-293-T Cells, HEK-293T Cells, HEK293T Cells, Human Embryonic Kidney 293T Cells

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About This Item

UNSPSC Code:
41106514
biological source:
human kidney (fetal)
growth mode:
adherent
morphology:
small slightly rounded cells growing in a dispersed manner
products:
SV40 T antigen
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Nombre del producto

293T cell line human, 12022001

biological source

human kidney (fetal)

growth mode

adherent

morphology

small slightly rounded cells growing in a dispersed manner

products

SV40 T antigen

shipped in

dry ice

storage temp.

−196°C

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General description

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line.[1] It expresses the large T antigen of simian virus 40 (SV40).[2]

Cell Line Description

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Application

293T cell line human has been used:
  • for lentiviral particle production[3]
  • in the expression of eukaryotic proteins[4][5]
  • for small interfering RNA (siRNA) transfection[6]
  • for human immunodeficiency virus (HIV) transfection for pseudoviruses production[7]
  • as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression[8]

Biochem/physiol Actions

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression.[2] It also possesses stem cell properties.[2] HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.[9]

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Culture Medium

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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  1. Denmark
    • Review 1
    • Votes 0
    2 out of 5 stars.

    Careful when odering! You might only order DNA, not viable cells!

    Extremely misleading product web page.
    As of today, a cell line is being described for sale, but they only sell extracted DNA. The whole web page is also listed under mammalian cell lines.
    Customer service clarified that viable cells have the product ID 12022001-1VL, for which there is no product entry (April 2024 to July 30th, 2024).
    So I waited for 2 months (!) to receive useless DNA.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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