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Merck

C1224

Sigma-Aldrich

Caspase 3 human

≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sinónimos:

Apopain, CPP32, Yama

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10 μG
MXP 13,438.00

MXP 13,438.00


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10 μG
MXP 13,438.00

About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32

MXP 13,438.00


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recombinante

expressed in E. coli (C-terminal histidine-tagged)

Nivel de calidad

Ensayo

≥85% (SDS-PAGE)

Formulario

buffered aqueous glycerol solution

actividad específica

≥1.0 units/mg protein

mol peso

~30 kDa

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... CASP3(836)

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Descripción general

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Aplicación

Caspase 3 human has been used to digest human r-vimentin.[1] It has also been used in caspase-3 inhibition assay[2] and protease inhibition assay.[3]

This product may also be used in cell-based apoptosis assay.

Acciones bioquímicas o fisiológicas

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.
Member of the CED-3 subfamily of caspases and responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation.

Definición de unidad

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

Forma física

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

Nota de preparación

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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