B9673

1-Bromo-3-chloropropane

for isolation of RNA

• Sinónimos: 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
• Fórmula lineal: Cl(CH₂)₃Br
• Número de CAS: 109-70-6
• Peso molecular: 157.44
• Beilstein: 605278
• Número CE: 203-697-1
• Número MDL: MFCD00000998
• Código UNSPSC: 41105515
• ID de la sustancia en PubChem: 24892149
• NACRES: NA.52
• bp: 144-145 °C (lit.)

Propiedades

• grado: Molecular Biology; for molecular biology
• Ensayo: ≥98.5% (GC)
• Formulario: liquid
• índice de refracción: n20/D 1.486 (lit.)
• bp: 144-145 °C (lit.)
• densidad: 1.592 g/mL at 25 °C (lit.)
• temp. de almacenamiento: room temp
• cadena SMILES: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
• Clave InChI: MFESCIUQSIBMSM-UHFFFAOYSA-N

Descripción

Descripción general

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA.[1] It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Aplicación

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth[2]
• mice brain and liver tissues[3]
• human ectocervical epithelial cells[4]
• placental samples[4]
• human mesenchymal stromal cells (hMSCs)[5]

Características y beneficios

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Otras notas

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Información de seguridad

• Pictogramas: GHS06,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H302,H331,H335,H341,H412
• Consejos de prudencia: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Clasificaciones de peligro: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 2
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter