A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Sinónimos: Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Fórmula empírica (notación de Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Número de CAS: 96087-38-6
• Peso molecular: 593.70
• Número MDL: MFCD00210085
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 24890604
• NACRES: NA.52

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• Ensayo: ≥95%
• Formulario: film or powder
• solubilidad: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• temp. de almacenamiento: −20°C
• cadena SMILES: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• Clave InChI: FFBLNTOMOSLSQM-AYEYRVMASA-N

Descripción

Descripción general

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.[1]

Aplicación

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization[2] to determine genomic relatedness.[3][4]

Acciones bioquímicas o fisiológicas

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.[1]

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins.[5] Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Precaución

Light sensitive.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)