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93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

Sinónimos:

20x SSC

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

product line

BioReagent

form

solution

technique(s)

Southern blotting: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.0±0.2

density

1.16 g/mL at 20 °C

storage temp.

room temp

General description

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Components

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Contenido relacionado

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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