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75259

Sigma-Aldrich

Tris(2-carboxietil)fosfina hydrochloride

BioUltra, ≥98% (NMR)

Sinónimos:

TCEP

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1 G
MXP 5,458.00
10 G
MXP 21,869.00

MXP 5,458.00

Fecha estimada de envío03 de abril de 2025

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1 G
MXP 5,458.00
10 G
MXP 21,869.00

About This Item

Fórmula empírica (notación de Hill):
C9H15O6P · HCl
Número de CAS:
51805-45-9
Peso molecular:
286.65
Beilstein/REAXYS Number:
3724376
MDL number:
MFCD00145469
UNSPSC Code:
12352128
PubChem Substance ID:
329766294
NACRES:
NA.25

MXP 5,458.00

Fecha estimada de envío03 de abril de 2025

Solicitar un pedido a granel

product line

BioUltra

Quality Level

300

assay

≥98% (NMR)

form

crystals

reaction suitability

reagent type: reductant

loss

≤1.0% loss on drying

pH

1.2-1.5

solubility

H2O: 1 M, clear, colorless (no residue)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.05 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

storage temp.

2-8°C

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

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General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution.[1][2] It readily and stoichiometrically reduces disulfides with high specificity.[1] TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.[2]

Application

Reactivo hidrosoluble, utilizado para reducción selectiva de disulfuros. Más estable que el DDT y útil en las aplicaciones de espectrometría de masas.
Tris(2-carboxyethyl)phosphine hydrochloride has been used:
  • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA[3]
  • in reducing sulfhydryl groups to obtain total thiol fraction[4]
  • to reduce protein samples[5]

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 1

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Certificados de análisis (COA)

Lot/Batch Number
BCCM1028
BCCK2560
BCCK9952
BCCH0850
BCCJ4621

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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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